Perbandingan Kualitas DNA Embrio Sawit (Elaeis guineensis Jacq) pada Ratio 260/230 dan 260/280

Mutiara Islami, Awalul Fatiqin, Pratiwi Erika

Sari


For the sustainability of world oil palm, it is necessary to conduct intensive breeding through genetic diversity studies in an effort to ensure the availability of planting material with high productivity. In plant breeding, it is necessary to check the quality of the DNA in the samples used, so this research was conducted to see the value of the purity of the embryonic DNA of oil palm ( Elaeis guineensis Jacq) at the ratio λ260 / 230 and λ260 / 280. This research was conducted at the Molecular Breeding laboratory of PT. Bina Sawit Makmur Palembang on 1-31 August 2019. DNA isolation of oil palm embryos was carried out using the KIT method (promega), with the stages of destruction (lysis), extraction, separation of DNA from solid materials such as cellulose and protein, and DNA purification. The results showed that at λ260 / 230 the purity values ranged from 0.47 to 3.039, 8 samples reached a purity value of 2-2.2, namely samples E2 3 , E2 5 , E2 8 , E2 9 , E2 12 , E2 18 , E2 37 and E2 42 . The highest purity value in sample E2 10with a value of 3.039, and the lowest value of 0.47 in the sample E2 16 . For embryonic DNA purity values at λ260 / 280 ranged from 0.719 to 1.806, only one sample reached a purity value of 1.8 - 2.0. From these results, it is known that there are still impurities and protein contaminants contained in the oil palm embryo DNA sample. This can occur due to the inaccurate speed of the extraction process and the deposition of several samples when taking supernatant / pellets per sample.

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Referensi


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P-ISSN (cetak): 2654-4032
E-ISSN (online): 2964-6820

Kantor Redaksi:
Fakultas Sains dan Teknologi
Universitas Islam Negeri Raden Fatah Palembang
email: semnassaintek_uin@radenfatah.ac.id
 

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